| distance | reference number | name | latitude | longitude |
| 0.4 | 973 | Fernandina Beach, Amelia River, Florida | 30.6717 | -81.465 |
| 2.2 | 1520 | Kingsley Creek, RR. bridge, Florida | 30.6317 | -81.4767 |
| 3.5 | 1667 | Lofton, Lanceford Creek, St. Marys River, Florida | 30.6433 | -81.5233 |
| 3.5 | 192 | Beach Creek ent., Cumberland Island, Georgia | 30.7267 | -81.4767 |
| 3.7 | 2928 | St. Marys Entrance, North Jetty, Georgia | 30.7167 | -81.4333 |
| 3.8 | 545 | Chester, Bells River, St. Marys River, Florida | 30.6833 | -81.5333 |
| 4.9 | 50 | Amelia City, South Amelia River, Florida | 30.5867 | -81.4633 |
| 5.6 | 2930 | St. Marys, St. Marys River, Florida | 30.72 | -81.5483 |
| 5.7 | 2716 | Seacamp Dock, Cumberland Island, Georgia | 30.7633 | -81.4717 |
| 6.5 | 1948 | Nassauville, St. Marys River, Florida | 30.5683 | -81.515 |
| 6.7 | 2590 | Roses Bluff, Bells River, St. Marys River, Florida | 30.7033 | -81.5767 |
| 8.1 | 1515 | Kings Bay, Georgia | 30.7967 | -81.515 |
| 8.1 | 713 | Cuno, Lofton Creek, St. Marys River, Florida | 30.5767 | -81.5717 |
| 8.4 | 1514 | Kings Bay NSB, Kings Bay, Cumberland Sound, Georgia | 30.8017 | -81.515 |
| 9.1 | 2929 | St. Marys River (entrance), Florida | 30.5183 | -81.4533 |
| 9.3 | 2692 | Sawpit Creek entrance, bridge, Florida | 30.5133 | -81.4567 |
| 9.6 | 677 | Crandall, St. Marys River, Florida | 30.7217 | -81.6217 |
| 9.9 | 2693 | Sawpit Creek, 1 mi. above entrance, Florida | 30.5033 | -81.4717 |
| 9.9 | 1198 | Halfmoon Island, highway bridge, St. Marys River, F | 30.575 | -81.6083 |
| 10.1 | 3093 | Tiger Point, Pumpkin Hill Creek, St. Marys River, F | 30.5017 | -81.495 |
| 10.3 | 1848 | Mink Creek entrance, St. Marys River, Florida | 30.5367 | -81.5817 |
| 10.5 | 691 | Crooked River, Cumberland Dividings, Georgia | 30.8433 | -81.4867 |
| 10.8 | 886 | Edwards Creek, 1 mi. above entrance, Florida | 30.5017 | -81.5417 |
| 12.4 | 2802 | Simpson Creek, A1A highway bridge, Florida | 30.465 | -81.4317 |
| 12.5 | 295 | Boggy Creek, 2 mi. above entrance, St. Marys River, | 30.5883 | -81.6633 |