| distance | reference number | name | latitude | longitude |
| 28.6 | 2234 | Petersburg, Appomattox River, James River, Virginia | 37.2333 | -77.4 |
| 31.1 | 2459 | Puddledock Sand & Gravel, Appomattox River, Virgini | 37.2667 | -77.3717 |
| 36.6 | 572 | City Point (Hopewell), James River, Virginia | 37.3167 | -77.2667 |
| 37.4 | 546 | Chester, James River, Virginia | 37.3833 | -77.3833 |
| 37.5 | 237 | Bermuda Hundred, Virginia | 37.3333 | -77.2667 |
| 38.2 | 1442 | Jordan Point, James River, Virginia | 37.3167 | -77.2167 |
| 38.4 | 1519 | Kingsland Reach, James River, Virginia | 37.4 | -77.3833 |
| 38.8 | 1802 | Meadowville, James River, Virginia | 37.3833 | -77.3167 |
| 39.6 | 958 | Falling Creek entrance, James River, Virginia | 37.4333 | -77.4333 |
| 39.7 | 1252 | Haxall, James River, Virginia | 37.3667 | -77.25 |
| 40.1 | 714 | Curles, 1 mile north of, James River, Virginia | 37.4 | -77.3 |
| 40.6 | 3311 | Westover, James River, Virginia | 37.3167 | -77.15 |
| 40.8 | 2537 | Richmond Deepwater Terminal, James River, Virginia | 37.45 | -77.4167 |
| 41.8 | 3361 | Windmill Point, James River, Virginia | 37.3 | -77.1 |
| 42.5 | 3345 | Willcox Wharf, Charles City, James River, Virginia | 37.3167 | -77.1 |
| 43.7 | 1708 | Lower Rocketts, James River, Virginia | 37.5 | -77.4167 |
| 45.6 | 2536 | Richmond (river locks), James River, Virginia | 37.5333 | -77.4167 |
| 45.9 | 578 | Claremont, James River, Virginia | 37.2317 | -76.9483 |
| 46.0 | 2976 | Sturgeon Point, James River, Virginia | 37.3 | -77 |
| 49.0 | 776 | Dillard Wharf, James River, Virginia | 37.2 | -76.8667 |
| 50.3 | 976 | Ferry Point (bridge), Chickahominy River, Virginia | 37.2667 | -76.8833 |
| 51.8 | 1900 | Mount Airy, Chickahominy River, Virginia | 37.35 | -76.9167 |
| 52.9 | 2708 | Scotland, Virginia | 37.1833 | -76.7833 |
| 53.0 | 2707 | Scotland, James River, Virginia | 37.185 | -76.7833 |
| 53.4 | 1414 | Jamestown Island, James River, Virginia | 37.2 | -76.7833 |